Biometra TRobot II – Automated PCR Thermal Cycler (2024)

Biometra TRobot II – Automated PCR Thermal Cycler (1)
  • Ensured work success thanks to reproducible, reliable results in high quality
  • Win walk-away time thanks to the possibility of reliable unattended 24/7 operation
  • Convenient operation by dedicated software or via complete integration into scheduler software
  • Minimum downtime thanks to more than 30 years of experience in thermal cycler development and robust equipment design.

The automated thermal cycler Biometra TRobot II allows a high-throughput PCR workflow with 24/7 reliable, unattended operation with highest quality of results for routine PCR or incubation applications. It is ideal for molecular biology labs that face a large number of samples every day and need to rely on the analysis results of their valuable samples.

High Degree of Automation for More Efficiency

  • Space-saving modular concept
  • Direct access to the sample block from three sides
  • Trouble-free removal of plates thanks patent-pending plate lifting system
  • Gradient function for optimization of new protocols
  • Touch-sensitive safety frame at the lid for maximum user-safety
  • Robust design for 24/7 use in automated systems
  • Patent-pending automated Smart Tune Lid ensures uniform conditions in all sample positions

Download Product Information

Brochures

Title Language Info
Brochure Thermal Cycler Biometra TRobot II (EN) en PDF, 336 KB
Product Guide Consumables for PCR/qPCR (EN) en PDF, 5 MB
Dimensions Overview for Biometra TRobot II (EN) en PDF, 185 KB
Broschüre Biometra TMS Temperaturmesssystem (DE) de PDF, 1 MB
Brochure Biometra TMS Temperature Measuring System (EN) en PDF, 1 MB
Flyer Biometra TSuite Software (DE) de PDF, 2 MB
Flyer Biometra TSuite Software (EN) en PDF, 3 MB
Br NGS Library Preparation (EN) en PDF, 2 MB
Brochure Product Guide Life Science (EN) en PDF, 2 MB
Broschüre Produktführer Life Science (DE) de PDF, 2 MB

Product Highlights in Detail

Always best results

In addition to the typical main application, the polymerase chain reaction (PCR), the Biometra TRobot II is also suitable for all other molecular biological or chemical incubations in PCR plates. For example, the generation of libraries for Next Generation Sequencing (NGS) is an important step in a larger workflow where the automated thermal cycler can be used. This reduces the manual effort and the possibility of errors. The PCR cycler has outstanding heating and cooling rates and excellent temperature hom*ogeneity across the sample block for highly reproducible PCR results. For the setup of new protocols or for the transfer of existing protocols from other thermal cyclers it is sometimes necessary to make slight optimizations to the protocols. For this purpose the Biometra TRobot II includes a gradient function. With this function it is possible, to test different temperatures in the critical annealing step of the PCR in one run.

Fit for integration

In automated systems, compact components with the smallest possible footprint are particularly important. The Biometra TRobot II meets this requirement through its modular design with a compact PCR module that is placed directly on the automation deck, while the controller can be stored underneath the platform. Another important point in automation is that all components should be very robust and reliable. If one component fails, the entire workflow comes to a halt. With this background, we have developed a special, patent-pending mechanism for opening and closing the lid of the Biometra TRobot II, which is intended for permanent use. At the same time, the block design was designed to enable the gripper to access the sample block from three sides. This opens up many possibilities for positioning the thermal cycler on the deck.

Ease of work

A conveniently prepared library allows a fast integration into automation systems. In addition, powerful computer software, the Biometra TSuite, is available for direct control of the automated thermal cycler, which is helpful for first PCR runs in preparation for the actual application. Maximum user safety is ensured with a unique, touch-sensitive safety frame which immediately stops the lid closing in case of contact with a resistance. The smooth block surface without gaps is essential for easy and effective cleaning and decontamination. For stress-free work it is also important to keep the noise level low. The ventilation system has been designed to remain very quiet, even at high performance levels. This allows you to work next to Biometra TRobot II concentrated!

Products

Biometra TRobot II 384 G, 230 V

384 well block format for high-throughput applications in research and routine.

It is available as 100 V, 115 and 230 V.

Order number: 846-2-070-903

Biometra TRobot II 96 G, 230 V

96 well block format for standard applications in research and routine.

It is available as 100 V, 110 V and 230 V version.

Order number: 846-2-070-901

Biometra TRobot II 96 SG, 230 V

96 well silver block for highest demands in research and routine.

It is available as 100 V, 115 V and 230 V version.

Order number: 846-2-070-902

Biometra TRobot II Controller 230 V

Controller for Biometra TRobot II block modules.

It is available as 100 V, 115 and 230 V.

Order number: 846-2-070-904

Biometra TRobot II PCR Module 384 G

Biometra TRobot II PCR module with 384 well block with gradient function.

Order number: 846-070-923

Biometra TRobot II PCR Module 96 G

Biometra TRobot II PCR Module with 96 well aluminum block with gradient function

Order number: 846-070-921

Biometra TRobot II PCR Module 96 SG

Biometra TRobot II PCR Module with 96 well silver block with gradient function

Order number: 846-070-922

Technical Data

Integration software

Sample Block

Block capacity

Proposed sample volume

Maximum Heating

Average Heating

Maximum Cooling

Dimension (width x depth x height)

Average Cooling

Temperature uniformity

Gradient

Max./Min. gradient

Adjustable gradient range

Block exchange

Tempering method

Standby temperature

Temperature control mode

Adjustable temperature range

Temperature control accuracy

Lid type

Space requirement (width x depth x height)

Lid temperature

Contact pressure

Compatible sealing systems

Plate lifter

Consumable compatibility

Time increment

Temperature increment/decrement

Additional dimensions

Required clearance zone

Biometra TRobot II 384 G, 230 V
846-2-070-903

Biometra Library (dll file) for integration into an automation system's scheduler software Aluminum, special alloy 384 well microplate 5 - 25µL 2.4°C/s 2.2°C/s 1.8°C/s 208 mm x 260 mm x 206 mm 1.6°C/s +/- 0,15 °C at 55 °C after 15 s yes 18 °C/0,1°C 24 rows 20 °C to 99°C no Peltier elements Down to 4 °C Control Mode 3 - 99°C +/- 0,1°C Motorized lid with heating module, which is connected spring-loaded to the lid 208 mm x 332 mm x 291 mm 30 - 110°C Independent from the used consumable adjusted via software in the range of 4 kg to 12 kg. Typical standard settings: 8 kg to 10 kg Sealing foils and mats Motor-driven, direct compatible with full-skirted PCR plates.With optional Biometra TRobot II adapter frame also compatible with semi-skirted PCR plates with these dimensions: distance well tip to semi-skirt: 12 to 14 mm with 96-well aluminum block, 11 to 13 mm with 96 well silver block. 384 well PCR microplates 1 - 240s/cycle +/- 0,1 °C to 20 °C per cycle Controller (W x D x H, mm): 240 x 387 (444 incl. cable ports) x 139 PCR module: 20 cm at the rear side. When operating several units side by side, an additional 10 cm between the units. Controller: 10 cm at the rear side

Biometra TRobot II 96 G, 230 V
846-2-070-901

Biometra Library (dll file) for integration into an automation system's scheduler software Aluminum, special alloy 96 x 0,2 mL tubes, 96 well microplates or 8 well strips 5 - 50µL 4.0°C/s 3.7°C/s 3.1°C/s 208 mm x 260 mm x 206 mm 2.8°C/s +/- 0,20 °C at 55 °C after 15 s yes 24 °C/0,1°C 12 rows 10 °C to 99°C no Peltier elements Down to 4 °C Block Control 3 - 99°C +/- 0,1°C Motorized lid with heating module, which is connected spring-loaded to the lid 208 mm x 332 mm x 291 mm 30 - 110°C Independent from the used consumable adjusted via software in the range of 4 kg to 12 kg. Typical standard settings: 8 kg to 10 kg Sealing foils and mats Motor-driven, direct compatible with full-skirted PCR plates.With optional Biometra TRobot II adapter frame also compatible with semi-skirted PCR plates with these dimensions: distance well tip to semi-skirt: 12 to 14 mm with 96-well aluminum block, 11 to 13 mm with 96 well silver block. Low-profile and high-profile PCR plates with full-skirt, semi-skirt and without skirt 1 - 240s/cycle +/- 0,1 °C to 20 °C per cycle Controller (W x D x H, mm): 240 x 387 (444 incl. cable ports) x 139 PCR module: 20 cm at the rear side. When operating several units side by side, an additional 10 cm between the units. Controller: 10 cm at the rear side

Biometra TRobot II 96 SG, 230 V
846-2-070-902

Biometra Library (dll file) for integration into an automation system's scheduler software Silver block with gold coating 96 x 0,2 mL tubes, 96 well microplates or 8 well strips 5 - 100µL 5.0°C/s 4.9°C/s 4.8°C/s 208 mm x 260 mm x 209 mm 4.5°C/s +/- 0,15 °C at 55 °C after 15 s yes 30 °C/0,1°C 12 rows 10 °C to 99°C no Peltier elements Down to 4 °C Block Control 3 - 99°C +/- 0,1°C Motorized lid with heating module, which is connected spring-loaded to the lid 208 mm x 332 mm x 291 mm 30 - 110°C Independent from the used consumable adjusted via software in the range of 4 kg to 12 kg. Typical standard settings: 8 kg to 10 kg Sealing foils and mats Motor-driven, direct compatible with full-skirted PCR plates.With optional Biometra TRobot II adapter frame also compatible with semi-skirted PCR plates with these dimensions: distance well tip to semi-skirt: 12 to 14 mm with 96-well aluminum block, 11 to 13 mm with 96 well silver block. Low-profile and high-profile PCR plates with full-skirt, semi-skirt and without skirt 1 - 240s/cycle +/- 0,1 °C to 20 °C per cycle Controller (W x D x H, mm): 240 x 387 (444 incl. cable ports) x 139 PCR module: 20 cm at the rear side. When operating several units side by side, an additional 10 cm between the units. Controller: 10 cm at the rear side

Biometra TRobot II Controller 230 V
846-2-070-904

240 x 387 (444 incl. cable ports) x 139 (W x D x H, mm) 10 cm at the rear side

Biometra TRobot II PCR Module 384 G
846-070-923

208 x 260 x 209 (mm) 20 cm to the rear. When operating several units side by side, an additional 10 cm between the units.

Biometra TRobot II PCR Module 96 G
846-070-921

208 x 260 x 209 (mm) 20 cm to the rear. When operating several units side by side, an additional 10 cm between the units.

Biometra TRobot II PCR Module 96 SG
846-070-922

208 x 260 x 209 (mm) 20 cm to the rear. When operating several units side by side, an additional 10 cm between the units.

For more information about our products and possible variants, please refer to the technical documents or use our contact form.

Order Information

Order number

Description

846-2-070-903 Biometra TRobot II 384 G, 230 V, with 96 well aluminum block (0.2 mL) with gradient feature, controller, with signal and power supply cable and power cord, RS232 cable, tool for mechanical opening of the PCR module lid, Biometra TSuite application software (1 license) and Biometra Library for software integration, documentation (German, English)
846-2-070-901 Biometra TRobot II 96 G, 230 V, with 96 well aluminum block (0.2 mL) with gradient feature, controller, with signal and power supply cable and power cord, RS232 cable, tool for mechanical opening of the PCR module lid, Biometra TSuite application software (1 license) and Biometra Library for software integration, documentation (German, English)
846-2-070-902 Biometra TRobot II 96 SG, 230 V, with 96 well silver block (0.2 mL) with gradient feature, controller, with signal and power supply cable and power cord, RS232 cable, tool for mechanical opening of the PCR module lid, Biometra TSuite application software (1 license) and Biometra Library for software integration, documentation (German, English)
846-2-070-904 Biometra TRobot II Controller 230 V, with fixed signal and supply cables
846-070-923 Biometra TRobot II PCR Module 384 G, inclusive tool for mechanical opening of the lid in case of power failure
846-070-921 Biometra TRobot II PCR Module 96 G, inclusive tool for mechanical opening of the lid in case of power failure
846-070-922 Biometra TRobot II PCR Module 96 SG, inclusive tool for mechanical opening of the lid in case of power failure

Technology Highlights

Smart Tune Lid concept

The unique arc-shaped opening movement has many advantages: In addition to a low instrument height, the sample block is completely accessible for the gripper from three sides. The vertical closing of the lid with software adjustable pressure on the sample plate ensures completely uniform conditions in all sample positions regardless of the height of the plate and sealing material. This feature ensures best reproducibility of results. The particularly robust and patent-pending single-motor mechanism is ideally designed for 24/7 use.

Smart release of plates

For a workflow without interruptions it is very important that the sample plate can be removed smoothly from the block. Since plates can get stuck on the block after long incubation at different temperatures, the Biometra TRobot II contains a patent-pending system with four small motor-driven plate lifters. When the lid is opened, they gently lift the plate out of the block and are then immediately recessed. This unique system is compatible with all full-skirted plates. A simple adapter frame is available for half-skirted plates.

Accessories

Biometra TRobot II adapter frame for semi-skirted plates 846-070-924
USB2.0-to-RS232-adapter 846-070-925
Ethernet cable CAT 6 846-070-926

Software

Biometra TSuite

User-friendly software for operating thermal cyclers in a network

The Biometra TRobot II is completely controlled by external software – typically integrated into the robot software using corresponding driver files or via the Biometra TSuite computer software. This software offers a full range of functions for controlling the cycler, creating programs, managing the device and documenting its relevant states and actions in detail.

Encrypted data transmission ensures the integrity and security of your data. Various log files enable GMP-compliant use and, in the event of malfunction, help locate the cause of the error prior to a service visit. The number of addressable instruments is almost unlimited – up to 1,000 devices can be managed by the software.

The most important features at a glance:

  • Control of one or up to 1,000 thermal cyclers connected in a network
  • User-guided operation via context-sensitive editing fields and icons, thematically arranged in main areas and with dashboard view on the start screen
  • Create, edit, run and export programsLive monitoring of programs
  • Thermal cycler user management
  • Saving and exporting various log files
  • Performing a detailed thermal cycler self-test and readout of a service info file
  • Creating backup files
  • Data integrity ensured through encrypted data transfer
  • Languages: English, German, Chinese
  • Installation requirements: computer operating system Windows 8.1 or higher
  • Compatible with the thermal cycler series Biometra TOne, Biometra TAdvanced, Biometra TRIO, Biometra TRobot II
Product Required Included
Biometra TRobot II 384 G, 230 V (846-2-070-903)
Biometra TRobot II 96 G, 230 V (846-2-070-901)
Biometra TRobot II 96 SG, 230 V (846-2-070-902)
Biometra TRobot II PCR Module 384 G (846-070-923)
Biometra TRobot II PCR Module 96 G (846-070-921)
Biometra TRobot II PCR Module 96 SG (846-070-922)

Biometra TSuite - Single License

Order number: 846-070-001

Multiple thermal cyclers, one place: Thanks to the Biometra TSuite software you can intuitively manage, program, and monitor multiple thermal cyclers from the comfort of your desk - regardless of how many you have or where they are located.

The Biometra TSuite thermal cycler management software gives you various possibilities to control and monitor your networked thermal cyclers from a computer. In the dashboard display, you can keep track of all important, current events and access active thermal cyclers directly. Through other windows, you can access detailed information on individual devices, programs, documentation files and general configuration options.

Benefit from many advantages in your everyday laboratory operations:

  • Suitable for all current models: Biometra TOne, Biometra TAdvanced, Biometra TRIO, Biometra TRobot II
  • Unlimited number of devices: Connect as many of your Biometra thermal cyclers as you want
  • Flexible usage: Choose between a mobile single license or multiple network licenses.
  • Compatible with existing devices: After a simple firmware update, you can use its full functionality.
Product Required Included
Biometra TRobot II 384 G, 230 V (846-2-070-903)
Biometra TRobot II 96 G, 230 V (846-2-070-901)
Biometra TRobot II 96 SG, 230 V (846-2-070-902)
Biometra TRobot II Controller 230 V (846-2-070-904)
Biometra TRobot II PCR Module 384 G (846-070-923)
Biometra TRobot II PCR Module 96 G (846-070-921)
Biometra TRobot II PCR Module 96 SG (846-070-922)

Consumables

844-70039-0 384 Well PCR Plate, full-skirted, white, suitable for automation - 100 pcs
844-70039-10D 384 Well PCR Plate, full-skirted, white, suitable for automation - 10 pcs
844-70037-5D 96 Well PCR Plate (0.2 mL; HP), half-skirted, white - 5 pcs
844-70029-0 96 Well PCR Plate (0.2 mL; LP), full-skirted, white, suitable for automation - 100 pcs
844-70038-10S 96 Well PCR Plate (0.2 mL; LP), full-skirted, white - 10 pcs
844-70038-S 96 Well PCR Plate (0.2 ml; LP), full-skirted, white - 100 pcs
844-70037-0 96 Well PCR Plate (0.2 mL; HP), half-skirted, white - 100 pcs
844-70034-5D 96 Well PCR Plate (0.2 mL; HP), half-skirted, transparent - 5 pcs
844-70034-0 96 Well PCR Plate (0.2 mL; HP), half-skirted, transparent - 100 pcs
844-70035-10D 96 Well PCR Plate (0.2 mL; LP), full-skirted, transparent - 10 pcs
844-70035-0 96 Well PCR Plate (0.2 mL; LP), full-skirted, transparent - 100 pcs
844-70029-10D 96 Well PCR Plate (0.2 mL; LP), full-skirted, white, suitable for automation - 10 pcs

Downloads

Br NGS Library Preparation (EN)
Brochure Biometra TMS Temperature Measuring System (EN)
Brochure Product Guide Life Science (EN)
Brochure Thermal Cycler Biometra TRobot II (EN)
Broschüre Biometra TMS Temperaturmesssystem (DE)
Broschüre Produktführer Life Science (DE)
Dimensions Overview for Biometra TRobot II (EN)
Flyer Biometra TSuite Software (DE)
Flyer Biometra TSuite Software (EN)
Product Guide Consumables for PCR/qPCR (EN)
Technical Data Biometra TRobot II (DE)
Technical Data Biometra TRobot II (EN)

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Biometra TRobot II – Automated PCR Thermal Cycler (2024)

FAQs

How much does a PCR cycler cost? ›

How much do PCR machines & thermal cyclers cost? Prices for new systems generally range from $3,000 to $30,000, depending on the throughput, features, and capabilities of the equipment.

What does a thermal cycler PCR machine do? ›

A PCR machine—or a thermal cycler—is an instrument that amplifies target nucleic acid sequences into millions of copies via polymerase chain reaction. Thermal cyclers inherit their name because they regulate temperatures in a cyclical program.

What is the difference between a thermal cycler and a PCR? ›

The thermal cycler (also known as a thermocycler, PCR machine or DNA amplifier) is a laboratory apparatus most commonly used to amplify segments of DNA via the polymerase chain reaction (PCR).

What is automated thermal cycler? ›

Thermocyclers are integral to life science research. In molecular biology they are used for DNA sequencing, cloning, generation of probes, quantification of DNA and RNA, studying patterns of gene expression, detection of sequence-tagged sites, and many more techniques.

How much does a PCR device cost? ›

Pcr Machine
Company NameCurrencyPrice
Bio Aid Lab SolutionsINR1400000
K.D.CHEMICAL AND SCIENTIFIC WORKSINR1150000
M/S GYAN SCIENTIFIC TRADERS (I) PVT. LTD.INR1200000
BIOERA LIFE SCIENCES PRIVATE LIMITEDINR35000
2 more rows

How much is a PCR machine? ›

PCR machines will vary in price, ranging from around $3,000 to above $15,000 with higher-end models. Browse the catalog of thermocyclers below across leading suppliers and submit a quote for more information, including cost, specifications, and more.

Can you do PCR without a thermal cycler? ›

And, if you refuse to spend any money on a thermocycler, you can make one from a lightbulb and a fan. Moreover, if you have access to a 3D printer, you can make your own qPCR (real-time PCR) machine.

How much DNA do you need to start with to do PCR? ›

Template DNA

Nevertheless, the composition or complexity of the DNA contributes to optimal input amounts for PCR amplification. For example, 0.1–1 ng of plasmid DNA is sufficient, while 5–50 ng of gDNA may be required as a starting amount in a 50 µL PCR.

What ingredients are required by the thermal cycler? ›

These components include:
  • DNA template that contains the DNA region (target) to be amplified.
  • Two primers, which are complementary to the DNA regions at the 5' (five prime) or 3' (three prime) ends of the DNA region.
  • A thermostable DNA polymerase such as Taq polymerase .

What is the end goal of PCR? ›

Typically, the goal of PCR is to make enough of the target DNA region that it can be analyzed or used in some other way. For instance, DNA amplified by PCR may be sent for sequencing, visualized by gel electrophoresis, or cloned into a plasmid for further experiments.

What materials are needed for PCR? ›

Standard PCR reagents include a set of appropriate primers for the desired target gene or DNA segment to be amplified, DNA polymerase, a buffer for the specific DNA polymerase, deoxynucleotides (dNTPs), DNA template, and sterile water.

For what is PCR used? ›

PCR is also valuable in a number of laboratory and clinical techniques, including DNA fingerprinting, detection of bacteria or viruses (particularly AIDS), and diagnosis of genetic disorders.

Is a thermocycler the same as a PCR machine? ›

Real-time thermocyclers are used to quantify the number of copies of a specific sequence using signals generated by fluorescent probes. Digital PCR Systems - dPCR instruments separate the DNA sample into tens of thousands of partitions as microscopic droplets to be measured digitally.

What are the three steps of the thermal cycler? ›

Three steps of PCR─denaturation, annealing, and extension─as shown in the first cycle, and the exponential amplification of target DNA with repeated cycling.

What is thermal cycling used for? ›

Thermal cycling testing is performed to investigate the long-term stability of PCM for solar TES applications. In this section, the effect of thermal cycle testing on the properties of different organic, inorganic, eutectic, and composite PCMs has been discussed.

How much does PCR recruiter cost? ›

MONTHLY OPTION. PCRecruiter accounts start at around $85/user per month for a basic two-year contract.

What is the price of Thermo Fisher thermal cycler? ›

Thermo Fisher MiniAmp Thermal Cycler at Rs 150000 | Thermo Fisher Biology Equipment in Mumbai | ID: 20556435091.

Is PCR cost effective? ›

PCR-severe-only resulted in a 63% reduction in infections and a 44% reduction in deaths, but required >65-fold more tests/day with 4-fold higher costs. PCR-all-repeat had an ICER <$100,000/QALY only when Re ≥1.8. At all Re values, PCR-any-symptom was cost-saving compared to other strategies.

What is the cost of RT PCR Biorad? ›

Biorad CFX96 Real Time Pcr Machine, 0.2 mL, 96 wells at Rs 1400000 in Jaipur.

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